LZTR1 Knockout cell line (SNU-398)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

LZTR1 Knockout cell line (SNU-398) Order

Catalog#

YKO-H1002

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

LZTR1 Knockout cell line (SNU-398)

Species

Human

Gene Name

LZTR1

Gene ID

8216

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 1 (based on transcript LZTR1-221)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LZTR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SNU-398 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LZTR1 gene knockout LZTR1 Knockout cell line (SNU-398) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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